|
| Status |
Public on Feb 27, 2024 |
| Title |
Control ChIP-seq from whole organism (ENCLB716BJP) |
| Sample type |
SRA |
| |
|
| Source name |
Caenorhabditis elegans strain OP139 (wgIs139(ceh-27::TY1 EGFP 3xFLAG(P000007_F10); unc-119(+));unc-119(ed3)) mixed sex young adult (73 hours) at L1 larva starved after bleaching stage insertion targeting C. elegans ceh-27
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
cell_type: whole organism
biomaterial_type: whole organisms
biosample encode accession: ENCBS253FYV (SAMN40084690)
collection date: not applicable
geographic location: not applicable
encode accession: ENCBS253FYV
note: Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to CEH-27.
Sex: pooled male and female
dev stage: young adult
age: 73 hour
donor_id: ENCDO518ZHE
lab: Valerie Reinke, Yale
source: Valerie Reinke
worm synchronization stage: L1 larva starved after bleaching
link: ENCBS253FYV at ENCODE; https://www.encodeproject.org/ENCBS253FYV/
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
general protocol: https://www.encodeproject.org/documents/d89f19f3-31f8-4079-9635-5d3dd799471e/@@download/attachment/Reinke_Lab_ModENCODE_ChIP_protocol_%28revised%205-24-13%29_4_mg.pdf
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
https://www.encodeproject.org/experiments/ENCSR925LDI/
***************
biological replicate number: 2
technical replicate number: 1
description: This is the ChIP-seq control of transgenic worms expressing ceh-27-eGFP fusion proteins.
experiment encode accession: ENCSR925LDI
assay title: Control ChIP-seq
assembly: ce11
encode release date: 2018-06-12
lab: Kevin White, UChicago
library encode accession: ENCLB716BJP
fragmentation methods: sonication (Bioruptor generic)
size range: 200-800
|
| Data processing |
See GSM*_README.txt supplementary file linked below
|
| |
|
| Submission date |
Feb 25, 2024 |
| Last update date |
Feb 27, 2024 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL22765 |
| Series (1) |
| GSE258299 |
Control ChIP-seq from whole organism (ENCSR925LDI) |
|
| Relations |
| BioSample |
SAMN40084690 |
