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| Status |
Public on Feb 28, 2024 |
| Title |
Up_126_ZT22_1 |
| Sample type |
SRA |
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| Source name |
Heads
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| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: Heads
genotype: tim_up126
time point: ZT22
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| Growth protocol |
Male and female flies were entrained in 12:12 LD conditions for 4 days at 25°C, then collected at ZT2, ZT6, ZT10, ZT14, ZT18 and ZT22.
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
Trizol
TruSeq RNA
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
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| Data processing |
Fastq files were trimmed using Fastp in SE mode.
Reads were aligned against dm6 using STAR.
Aligned reads were sorted and indexed using samtools.
A count matrix was produced using featurecounts.
A normalized counts table was produced using DESeq2.
Assembly: dm6
Supplementary files format and content: tab-delimited text file with raw counts for each sample and a csv file with normalized counts for each sample
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| Submission date |
Feb 26, 2024 |
| Last update date |
Feb 28, 2024 |
| Contact name |
Albert Dayuan Yu |
| E-mail(s) |
albert.dayuan.yu@gmail.com
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| Organization name |
Brandeis University
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| Lab |
Rosbash Lab
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| Street address |
415 South St.
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| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
02453 |
| Country |
USA |
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| Platform ID |
GPL22106 |
| Series (1) |
| GSE259245 |
RNA-Seq of Drosophila tim promoter/enhancer mutants around the clock |
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| Relations |
| BioSample |
SAMN40137392 |
| SRA |
SRX23739853 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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