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Sample GSM8118015 Query DataSets for GSM8118015
Status Public on Mar 02, 2024
Title Subject 8 Day 23/24
Sample type protein
 
Source name Serum/Plasma
Organism Homo sapiens
Characteristics tissue: SLE Patient
condition: Serum/Plasma
Treatment protocol NA
Growth protocol NA
Extracted molecule protein
Extraction protocol Blood was collected from SLE patients and healthy controls and then processed into serum or plasma which was then screened by array.
Label R-PE conjugated goat anti-human IgG secondary antibody
Label protocol Decondary antibody conjugated to a fluorophore was added to a PBS solution containing conjugated beads bound to antibodies from samples.
 
Hybridization protocol A fluorescent secondary antibody was added to a PBS solution containing conjugated beads bound to antibodies.
Scan protocol A Luminex FlexMAP 3D instrument was used to quantify autoantibody levels in each sample by measuring fluorescence (in MFI) from each barcoded bead.
Description Antibodies in samples bind to barcoded beads conjugated to antigens. Antibody reactivity was quantified by the fluorescence measured as MFI from the secondary antibody.
Data processing Barebeed MFI values were subtracted from the MFI values for all analytes in each sample and replicate MFI values were averaged.
CTD Array version 2 Platform Raw MFI_02272024.xlsx: raw data
matrix_MFI.txt: Numerical values represent median fluorescent intensity (MFI).
 
Submission date Feb 28, 2024
Last update date Mar 02, 2024
Contact name Allan Feng
E-mail(s) afeng1@stanford.edu
Phone 2055678956
Organization name Stanford School of Medicine
Department Imm/Rheum
Lab Utz
Street address 269 Campus Dr
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL34257
Series (1)
GSE260478 Impaired innate and adaptive immune responses to BNT162b2 SARSCoV-2 vaccination in systemic lupus erythematosus [CTD]

Supplementary data files not provided

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