|
| Status |
Public on Mar 16, 2012 |
| Title |
Autoantibody profiling of normal sample 2 |
| Sample type |
other |
| |
|
| Source name |
Human Blood
|
| Organism |
Homo sapiens |
| Characteristics |
individual: healthy control
tissue: Plasma/Serum
|
| Treatment protocol |
Precleared Plasma/serum samples were diluted in Washing Buffer and applied to the protein array for the autoantibodyome profiling. The probed protein microarrays were dried and scanned with the Genepix 4200AL Microarray Scanner the Genepix Pro 6.1 software. All probing conditions and procedure was followed as described in invitrogen website. (http://tools.invitrogen.com/content/sfs/manuals/protoarrayIRBP_short_protocol.pdf)
|
| Extracted molecule |
other |
| Extraction protocol |
Precleared Plasma/serum samples were diluted in Washing Buffer and applied to the protein array
|
| Label |
Alexa Fluor 647
|
| Label protocol |
All probing conditions and procedure was followed as described in invitrogen website. (http://tools.invitrogen.com/content/sfs/manuals/protoarrayIRBP_short_protocol.pdf)
|
| |
|
| Hybridization protocol |
All probing conditions and procedure was followed as described in invitrogen website. (http://tools.invitrogen.com/content/sfs/manuals/protoarrayIRBP_short_protocol.pdf)
|
| Scan protocol |
The probed protein microarrays were dried and scanned with the Genepix 4200AL Microarray Scanner the Genepix Pro 6.1 software.
|
| Description |
MDS_N02_0000022633.gpr
|
| Data processing |
The arrays were scanned to obtain signal location, intensity quantification and identification information using GenePix Pro 6.1. For each array inter-array normalization was performed via the ProCAT algorithm (sliding window of length 15). The arrays were then quantile normalized and a comparison of intensities of probes was carried out between the subject and the healthy control group using a two-tailed Mann-Whitney non-parametric test, P<0.01, in Mathematica 8.0 and using Benjamini-Hochberg to correct for multiple hypothesis tests, adjusted P<0.01.
|
| |
|
| Submission date |
Oct 07, 2011 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Hogune Im |
| Phone |
650-723-3277
|
| Organization name |
Stanford
|
| Department |
Genetics
|
| Lab |
Snyder
|
| Street address |
300 Pasteur Dr. Alway M308
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL14689 |
| Series (2) |
| GSE32691 |
Autoantibody profile timecourse of UNK |
| GSE33029 |
Integrated analysis of omics profiles |
|
