|
Status |
Public on Oct 07, 2013 |
Title |
H1299 miR-4423 overexpression 2 |
Sample type |
RNA |
|
|
Source name |
H1299 cancer cell line
|
Organism |
Homo sapiens |
Characteristics |
condition: Empty vector control batch: 1
|
Treatment protocol |
Plasmids containing empty vector as a control were transfected using Lipofectamine 2000 (Invitrogen) as per manufacturer’s protocol.
|
Growth protocol |
H1299 cells were cultured in RPMI 1640 growth medium (ATCC) and plated at a 50% confluence in 60mm plates 24h before transfection.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using the miRNeasy mini kit (Qiagen) as per manufacturer’s instructions.
|
Label |
Biotin
|
Label protocol |
The total RNA was converted to cDNA and subsequently amplified and biotin-labeled.
|
|
|
Hybridization protocol |
cDNA was fragmented end-labeled with a biotinylated dideoxynucleotide using terminal transferase and added to a hybridization cocktail, loaded on a Human Gene1.0 ST GeneChip and hybridized for 16 hours at 45 ºC under rotation (60 rpm). Following hybridization, the array was washed and stained according to the standard Affymetrix protocol.
|
Scan protocol |
Arrays were scanned using an Affymetrix GeneChip Scanner 3000. These scans were used to generate CEL files for each array.
|
Data processing |
Transcript-level gene expression estimates were generated via the robust multichip average (RMA) algorithm within each batch using the Entrez Gene CDF v11 (http://brainarray.mbni.med.umich.edu) in R v2.9.2.
|
|
|
Submission date |
Oct 11, 2011 |
Last update date |
Oct 07, 2013 |
Contact name |
Avrum Spira |
E-mail(s) |
aspira@bu.edu
|
Organization name |
Boston University
|
Department |
Computational Biomedicine
|
Lab |
Spira/Lenburg Lab
|
Street address |
72 East Concord Street, E631
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02118 |
Country |
USA |
|
|
Platform ID |
GPL13243 |
Series (2) |
GSE48796 |
Identification of miR-4423 as a Primate-Specific microRNA Highly Expressed in Ciliated Airway Epithelium and Associated with Lung Cancer [Affymetrix] |
GSE48798 |
Identification of miR-4423 as a Primate-Specific microRNA Highly Expressed in Ciliated Airway Epithelium and Associated with Lung Cancer |
|