|
| Status |
Public on Jul 24, 2012 |
| Title |
HMEC-hTert-Zeb2+Ras |
| Sample type |
RNA |
| |
|
| Source name |
HMEC, hTert, Zeb2+Ras
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HMEC-derivatives
cell type: expressed hTert, Zeb2 and HRasG12V
|
| Growth protocol |
Cells were grown in DMEM/HAMF12 medium supplemented with penicillin/strptomycin, L-glutamin, 10% fetal bovine serum, 10ng/ml hEGF, 0.5µg/ml hydrocortisone and 10µg/ml insulin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extract with with the Qiagen RNeasy minikit (Qiagen), then 100 ng of total RNAs were amplified using Kit GeneChip 3’ IVT Express (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
According to manufacturer's instructions (Affymetrix).
|
| |
|
| Hybridization protocol |
According to manufacturer's instructions (Affymetrix).
|
| Scan protocol |
The Arrays were scanned using the Gene Chip Scanner 3000. Then CEL files were generated using the Affymetrix GeneChip Command Console software (AGCC) 3.0.
|
| Description |
transformed
|
| Data processing |
CHP files were processed using RMA analysis algorithm with Affymetrix Expression Console software 1.1.
|
| |
|
| Submission date |
Oct 11, 2011 |
| Last update date |
Jul 24, 2012 |
| Contact name |
stéphane ansieau |
| E-mail(s) |
ansieau@lyon.fnclcc.fr
|
| Phone |
0033-478785160
|
| Fax |
0033-478782821
|
| Organization name |
Inserm U590
|
| Department |
Centre Léon Bérard
|
| Street address |
28 rue Laennec
|
| City |
Lyon |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE32727 |
EMT inducers catalyze malignant transformation of mammary epithelial cells and drive tumorigenesis towards claudin-low tumors [human] |
| GSE32905 |
EMT inducers catalyze malignant transformation of mammary epithelial cells and drive tumorigenesis towards claudin-low tumors |
|
