|
| Status |
Public on Mar 09, 2024 |
| Title |
300rep3C |
| Sample type |
SRA |
| |
|
| Source name |
oocyte
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
cell type: oocyte
treatment: 300mM NaCl culture
|
| Treatment protocol |
adolescent C. elegans mothers were transferred to NGM with a 300mM NaCl concentration until day 1 of adulthood
|
| Growth protocol |
animals were raised on NGM under standard culture conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
C. elegans mothers were cultured from the adolescent L4 stage until the first day of adulthood on control NGM culture or on osmotic stress 300mM NaCl culture. The mature oocytes were then dissected and collected in SMART seq buffer.
SMARTer Stranded Total RNA-Seq Kit v3 - Pico
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Takara's cogentAP
STAR alignment
kallisto pseudo alignment (QC)
Assembly: WBcel235
Supplementary files format and content: comma separated value (CSV) containing differentially expressed genes (e.g. significance, fold change) of transcriptomes between treatment groups
|
| |
|
| Submission date |
Mar 08, 2024 |
| Last update date |
Mar 09, 2024 |
| Contact name |
Robert Fillinger |
| E-mail(s) |
robert.fillinger@vai.org
|
| Organization name |
Van Andel Institute
|
| Street address |
333 Bostwick Ave NE
|
| City |
Grand Rapids |
| State/province |
Michigan |
| ZIP/Postal code |
49503 |
| Country |
USA |
| |
|
| Platform ID |
GPL26672 |
| Series (1) |
| GSE261225 |
Oocyte mitochondria link maternal environment to offspring phenotype in C. elegans |
|
| Relations |
| BioSample |
SAMN40344575 |
| SRA |
SRX23885928 |
