|
| Status |
Public on Oct 16, 2011 |
| Title |
ID3 wild type mouse CD8+ T cells, pooled sample#1, gene-level |
| Sample type |
RNA |
| |
|
| Source name |
CD8+ T cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: Wild type
batch: Array batch 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNEasy Micro kit (Qiagen), following manufatcturer's instructions
|
| Label |
biotin
|
| Label protocol |
WT expression kit (Ambion), WT Terminal Labeling Kit (Affymetrix), according to the manufatcturer's instructions
|
| |
|
| Hybridization protocol |
Samples were hybridized to WT Human Gene 1.0 ST arrays using Affymetrix hybridization kit materials, according to the manufacturrer's instructions. Sample cocktails were heated at 99°C for 5 minutes, then 45°C for 5 minutes, centrifuged at max speed for 1 minute. Eighty ul of hyb solution was transferred to each array, and hybridized 17 hours at 45°C at 60rpm. Fluidics washing protocol used was FS450_0007.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
ID3 WT- 1
WT-6_(MoGene-1_0-st-v1).CEL
mouse ID3+/+ CD8+ T cells, pooled sample derived from four mice, sample #1
|
| Data processing |
Data from .CEL files were imported into Partek Genomincs Suite using the RMA method. For gene level analyses, probeset level data were merged using the median probeset expression level.
probe group file: MoGene-1_0-st-v1.r4.pgf
meta-probeset file: MoGene-1_0-st-v1.r4.mps
|
| |
|
| Submission date |
Oct 11, 2011 |
| Last update date |
Oct 16, 2011 |
| Contact name |
Zoltan Pos |
| E-mail(s) |
pos.zoltan@outlook.com.com
|
| Phone |
+(36)12102930-56435
|
| Organization name |
Semmelweis University
|
| Department |
Dept. of Genetics, Cell- and Immunobiology
|
| Street address |
4 Nagyvarad ter
|
| City |
Budapest |
| ZIP/Postal code |
H-1089 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE23568 |
Expression data analyzing ID3 (Inhibitor of DNA binding 3)-affected mouse T cells |
|
| Relations |
| Alternative to |
GSM813926 (exon-level analysis) |
