|
| Status |
Public on Mar 14, 2024 |
| Title |
Sample 3, Non-Immunized |
| Sample type |
SRA |
| |
|
| Source name |
PBMC
|
| Organism |
Cavia porcellus |
| Characteristics |
cell type: PBMC
genotype: Non-Immunized
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Rneasy mini plus kit (Qiagen). 1.5ug of total RNA was used for building sequencing libraries.
RNA libraries for RNA-Seq were prepared using TruSeq Ribo-Zero Total RNA Library preparation Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
4R123-L2-P10-TAGCTT
|
| Data processing |
Trimmed sequencing reads were mapped to Cavpor3.0 and feature count data was generated using htSeq.
Assembly: Cavpor3.0
Supplementary files format and content: Tab-delimited text file includes raw counts for each sample.
Supplementary files format and content: Tab-delimited text file includes RPKM values for each sample.
|
| |
|
| Submission date |
Mar 14, 2024 |
| Last update date |
Mar 14, 2024 |
| Contact name |
Lbachir BenMohamed |
| Organization name |
University of California Irvine
|
| Street address |
843 Health Sciences Road, Hewitt Hall, 2nd Floor
|
| City |
Irvine |
| State/province |
California |
| ZIP/Postal code |
92617 |
| Country |
USA |
| |
|
| Platform ID |
GPL32213 |
| Series (1) |
| GSE261599 |
Therapeutic Prime/Pull Vaccination of HSV-2 Infected Guinea Pigs with the Ribonucleotide Reductase 2 (RR2) and CXCL11 boosts TRM and TEM CD4+/CD8+ T cells to protect from recurrent genital herpes |
|
| Relations |
| BioSample |
SAMN40460231 |
| SRA |
SRX23952676 |
