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Sample GSM814749 Query DataSets for GSM814749
Status Public on Oct 03, 2012
Title Gfi1KO_thymocytes-2
Sample type RNA
 
Source name thymocytes
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: Gfi1 KO
protocol: untreated
Treatment protocol Overall procedure: Gfi1f/f (WT_Tum) or Mx1Cre+Gfi1f/f (KO_Tum) or Gfi1f/f Notch tg (WT_Notch_Tum) or Mx1Cre+Gfi1f/f Notch tg (Notch_KO_Tum)Mice were injected with di-Nitriso-N-Ethylurea (ENU) and observed for emergence of mediastinal using ultarsound. Upon emergence of a growing mediastinal tumor, mice were injected with pIpC, which induces deletion of Gfi1 in MxCre+ tg mice. About 2-3 weeks after pIpC injection, tumor cells were explanted.
Extracted molecule total RNA
Extraction protocol Quiagen micro-RNA kit was used
Label biotin
Label protocol Target RNA was reverse transcribed into cDNA using a NuGEN Ovation Pico RNA Kit and in-vitro transcription was performed to generate biotin-labeled cRNA. cRNA was labeled with streptavidin-phycoerythrin.
 
Hybridization protocol Samples were hybridized with [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Arrays and scanned using a GeneArray Scanner at the Génome Québec INNOVATION centre of the McGill University, Montréal, QC, Canada
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description Gfi1KO_thymocytes-080.CEL
Data processing Data was processed using the AltAnalyze_V.2.0.3 Win32 software
 
Submission date Oct 12, 2011
Last update date Oct 03, 2012
Contact name Lothar Vassen
E-mail(s) lothar.vassen@ircm.qc.ca
Phone 514-987-3351
Organization name IRCM
Department Hematopoiesis and Cancer
Lab Dr. Möröy
Street address 110, avenue des Pins Ouest
City Montréal
State/province Quebec
ZIP/Postal code H2W 1R7
Country Canada
 
Platform ID GPL10740
Series (1)
GSE32910 Growth factor independence 1 (Gfi1) as a new target for leukemia therapy

Data table header descriptions
ID_REF
VALUE log2 scale GC-RMA signal values

Data table
ID_REF VALUE
10344615 8.06559
10344617 3.79934
10344625 10.11925
10344626 12.14279
10344627 9.79458
10344628 11.95874
10344629 10.06164
10344630 11.04033
10344631 11.99192
10344632 12.32381
10344634 9.99725
10344636 10.32027
10344639 11.11598
10344640 12.36758
10344641 10.36175
10344642 9.63624
10344643 8.00939
10344644 9.38861
10344645 10.21819
10344646 9.39945

Total number of rows: 182058

Table truncated, full table size 3064 Kbytes.




Supplementary file Size Download File type/resource
GSM814749.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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