|
| Status |
Public on Mar 19, 2024 |
| Title |
180-Air-PBS-5 US84403582_252800518903_S01_GE1_107_Sep09_2_4 |
| Sample type |
RNA |
| |
|
| Source name |
lung
|
| Organism |
Mus musculus |
| Characteristics |
animal #: 60
Sex: female
exposure days: 180
smoking or air: Air
strain: PBS
day: 5
strain: C57BL/6j mouse
|
| Treatment protocol |
Seven-week-old female C57BL/6j mice were purchased from Charles River Japan Inc. Mice were exposed to cigarette smoke (Peace®, 28 mg of tar, 2.3 mg of nicotine/cigarette, Japan Tobacco Inc., Tokyo, Japan) or to air (as a control) for 20‒30 min per time by using a mainstream smoke-generating apparatus for period of 30, 90, or 180 days. Following smoke exposure, mice were intranasally inoculated with A(H7N9) virus (refered to as the Anhiu strain). For controls, mice were mock-infected with sterile PBS-solution. Lungs were harvested at days 2 or 5 post infection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from lung samples by using the RNeasy Mini Kit (Qiagen), according to the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
Using the Quick Amp Labeling Kit, T7 RNA polymerase was used to simultaneously amplify target material and incorporate cyanine 3-labeled CTP to prepare Cy3-labeled cRNA.
|
| |
|
| Hybridization protocol |
Using Agilents Gene Expression Hybridizatoin kit #4 and Gene Expression wash pack #5, Cy3-labeled cRNA preparations were hybridized with mouse arrays (Agilent Microarray Design Identification Number 028005) for 17 h at 65°C.
|
| Scan protocol |
Immediately after washing, Feature Extraction Software, version 7 (Agilent Technologies) was used for image analysis and data extraction.
|
| Description |
180-Air-PBS-5
|
| Data processing |
Per chip and per gene normalization was performed using the LIMMA package in R.
|
| |
|
| Submission date |
Mar 14, 2024 |
| Last update date |
Mar 19, 2024 |
| Contact name |
Yoshihiro Kawaoka |
| Organization name |
Japanese Science and Technology Agency
|
| Department |
ERATO Influenza-induced Host Responses Project
|
| Lab |
Kawaoka
|
| Street address |
Shirokanedai 4-6-1
|
| City |
Minato |
| State/province |
Tokyo |
| ZIP/Postal code |
1088639 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE261627 |
Attenuation of A(H7N9) influenza virus infection in mice exposed to cigarette smoke |
|
