|
| Status |
Public on Dec 15, 2011 |
| Title |
genomic DNA of HCT116 WT cells (r3) |
| Sample type |
genomic |
| |
|
| Source name |
Colon
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT116
genotype: wild type
|
| Growth protocol |
HCT116 cells were maintained in McCoy's 5A medium (Gibco BRL) supplemented with 10% fetal bovine at 37°C under 5% CO2.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted and purified from HCT116 cells using Qiagen QiaAMP Kit according to standard instructions. The recommended RNase treatment was performed.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Infinium HD Methylation Assay protocol
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Infinium HD Methylation Assay protocol.
|
| Scan protocol |
Arrays were imaged using HiScan Reader using standard recommended Illumina scanner setting.
|
| Description |
Colorectal cancer cell line
Sample_3.AVG_Beta
|
| Data processing |
GenomeStudio software v2010.3, Methylation module v1.8.5
|
| |
|
| Submission date |
Oct 12, 2011 |
| Last update date |
Dec 15, 2011 |
| Contact name |
Matthieu Defrance |
| Organization name |
Université Libre de Bruxelles
|
| Lab |
Cancer Epigenetics
|
| Street address |
CP614 route de Lennik 808
|
| City |
Bruxelles |
| ZIP/Postal code |
1070 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE29290 |
Evaluation of the Infinium Methylation 450K technology |
|
