|
| Status |
Public on Mar 21, 2024 |
| Title |
apocrine gland anal sac adenocarcinoma (AGASAC), sublumbal lymph node metastasis from 3 [a4.2] |
| Sample type |
RNA |
| |
|
| Source name |
archival FFPE tumour tissue
|
| Organism |
Canis lupus familiaris |
| Characteristics |
tissue: FFPE tumour tissue
animal: male, neutered
breed: Mixed-breed
dv200 (%): 79.7
age (years): 10
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted utilising the PureLinkTM FFPE Total RNA Isolation Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA).
|
| Label |
not provided
|
| Label protocol |
not provided
|
| |
|
| Hybridization protocol |
150 to 250 ng of total RNA was hybridised to the nCounter Canine IO Panel XT CodeSets (NanoString Technologies, Inc., Seattle, WA, USA). Hybridised samples were processed following the manufacturer’s protocols (protocol ID: MAN-10023-11, MAN-10056-06) and loaded onto the nCounter MAX Analysis System’s (NanoString) Prep Station for purification and immobilization on sample cartridges.
|
| Scan protocol |
Samples were transferred to the nCounter MAX Digital Analyzer (NanoString) for data collection following the manufacturer’s user manual (protocol ID: MAN-C0035-08).
|
| Description |
a4.2
|
| Data processing |
Following the workflow described in the manufacturer’s user manuals (MAN-C0019-08, MAN-C0011-04), the reporter library files (RLF) and reporter code count (RCC) files were imported into the nSolver 4.0 Analysis Software. Quality control and normalisation followed default settings.
|
| |
|
| Submission date |
Mar 18, 2024 |
| Last update date |
Mar 21, 2024 |
| Contact name |
Alexander F. H. Haake |
| E-mail(s) |
alexander.haake@fu-berlin.de
|
| Organization name |
Freie Universität Berlin
|
| Department |
Department of Veterinary Medicine
|
| Lab |
Institute of Veterinary Pathology
|
| Street address |
Robert-von-Ostertag-Straße 15
|
| City |
Berlin |
| ZIP/Postal code |
14163 |
| Country |
Germany |
| |
|
| Platform ID |
GPL34305 |
| Series (1) |
| GSE261791 |
Strong Correlation of Gene Counts and Differentially Expressed Genes between a 3′ RNA-Seq and RNA Hybridisation Platform from Canine Archival Tissue [nCounter] |
|
