|
| Status |
Public on Mar 20, 2024 |
| Title |
IMM cells, 60 minute LPS Stimulation Rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
IMM, 60 minute LPS Stimulation
|
| Organism |
Mus musculus |
| Characteristics |
cell type: IMM
|
| Treatment protocol |
After 5 passages following thaw from -80C in cDMEM, LPS (Enzo Life Sciences) was used to stimulate the cells (100 ng / mL in cDMEM) at the 0, 30, 60, and 120 minute timepoints. After stimulation the plates (10 cm treated dishes) had the LPS containing media aspirated off the top and were immediately placed on ice. 2 washes with ice cold PBS were performed before the addition of lysis buffer.
|
| Growth protocol |
IMM cells maintained in cDMEM. DMEM supplemented with heat inactivated 10% FBS and 0.02M HEPES in a humidified atmosphere with 5% CO2 and 37C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested with the miRNeasy mini plus kit (Qiagen)
|
| Label |
Nanostring
|
| Label protocol |
n/a
|
| |
|
| Hybridization protocol |
Hybridization was carried out according to the NanoString manufacturer Guidelines.
|
| Scan protocol |
Scanning was carried out by the NCI CCR Genomics core according to the NanoString manufacturer Guidelines.
|
| Data processing |
Raw files and the RLF were imported into nSolver Analysis 4.0. Normalization was performed relative to the unstimulated 0 minute timepoint using the Normalize function provided in the software.
|
| |
|
| Submission date |
Mar 18, 2024 |
| Last update date |
Mar 20, 2024 |
| Contact name |
Matthew James Marino |
| E-mail(s) |
matthew.marino@nih.gov
|
| Phone |
301-761-6981
|
| Organization name |
NIH
|
| Department |
NIAID
|
| Lab |
LISB
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20815 |
| Country |
USA |
| |
|
| Platform ID |
GPL34308 |
| Series (1) |
| GSE261854 |
Effect of stimulation with lipopolysaccharide (LPS) at 0,30, 60, and 120 minutes on miRNA expression in immortalized mouse macrophages (IMM). |
|
