|
| Status |
Public on Nov 15, 2011 |
| Title |
H295R 100uM 8Br-cAMP 24h replicate 3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
H295R 100uM 8Br-cAMP 24h replicate 3
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H295R (CRL-2128)
treatment: 100uM 8Br-cAMP
time: 24h
|
| Treatment protocol |
8Br-cAMP dissolved in MilliQ water, blanks are solvent control samples, treatment in routine cell culture medium
|
| Growth protocol |
H295R cells grown routinely in DMEM/F12 supplemented with 1% ITS-G and 2% UltroserSF serum; split 1/3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using the Qiagen RNeasy mini kit
|
| Label |
Cy5
|
| Label protocol |
Agilent Low RNA Input Amplification Kit (LRILAK) protocol
|
| |
|
| Channel 2 |
| Source name |
H295R Reference pool
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H295R (CRL-2128)
treatment: reference
|
| Treatment protocol |
8Br-cAMP dissolved in MilliQ water, blanks are solvent control samples, treatment in routine cell culture medium
|
| Growth protocol |
H295R cells grown routinely in DMEM/F12 supplemented with 1% ITS-G and 2% UltroserSF serum; split 1/3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using the Qiagen RNeasy mini kit
|
| Label |
Cy3
|
| Label protocol |
Agilent Low RNA Input Amplification Kit (LRILAK) protocol
|
| |
|
| |
| Hybridization protocol |
Agilent Gene Expression Hybridization protocol
|
| Scan protocol |
scanned using a Genetix Personal 4100A scanner; feature extration using the GenePix Pro analysis software 4.1
|
| Data processing |
VSN normalization
|
| |
|
| Submission date |
Oct 12, 2011 |
| Last update date |
Nov 15, 2011 |
| Contact name |
Caroline Vanparys |
| E-mail(s) |
caroline.vanparys@ua.ac.be
|
| Organization name |
University of Antwerp
|
| Lab |
EB&T
|
| Street address |
Groenenborgerlaan 171
|
| City |
Antwerp |
| ZIP/Postal code |
2020 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL9718 |
| Series (1) |
| GSE32926 |
Transcriptional profiling of 8Br-cAMP treated human H295R adrenal cortex |
|
