|
| Status |
Public on Apr 24, 2024 |
| Title |
GAD2_ABA_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis_GAD2_ABA_rep1
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: leaf
age: 6-8 week old
genotype: GAD2
|
| Treatment protocol |
Leaves were sprayed with 50 µM +/- ABA containing 25 µM biologically active ABA and 0.01 % (v/v) Triton X-100 for 4h before harvested
|
| Growth protocol |
Arabidopsis plants grow in soil in a 12 -h day–night rhythm (22/16 °C, 60% RH) and were illuminated with 125 μmol m−2 s−1 white light
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNeasy Plant Mini Kit (www.quiagen.com) according to the manufacturers protocol.
|
| Label |
Cy3
|
| Label protocol |
Total 0.5μg RNA was used for each sample labelling. Labelling and preparation of samples for hybridization was performed as described in the one-colour microarray-based gene-expression analysis protocol provided by Agilent, including the one-colour RNA spike-in kit (v.5.0.1, 2006; Agilent Technologies).
|
| |
|
| Hybridization protocol |
Cya 3-labeled, linearly amplified cRNA was incubated at 60°C for exactly 30 minutes to fragment RNA
|
| Scan protocol |
Slides were scanned on the Agilent Microarray Scanner with extended dynamic range at high resolution (5 μm).
|
| Description |
Oct12_1_1_252116915275
|
| Data processing |
Datasets were extracted by propriety software package (v.9.5.3.1, Agilent Technologies) using a standard protocol. Data preprocessing was performed using Bioconductor software with the statistical programming environment R.
Normalization has been performed using negative control probes and quantile normalization using negative and positive control probes as implemented in the neqc function of the limma package
|
| |
|
| Submission date |
Mar 20, 2024 |
| Last update date |
Apr 24, 2024 |
| Contact name |
Bo Xu |
| E-mail(s) |
b.xu@adelaide.edu.au
|
| Organization name |
University of Adelaide
|
| Street address |
Plant Research Centre
|
| City |
Glen Osmond |
| ZIP/Postal code |
5072 |
| Country |
Australia |
| |
|
| Platform ID |
GPL12621 |
| Series (1) |
| GSE261985 |
GABA does not regulate stomatal CO2 signalling in Arabidopsis |
|
