|
| Status |
Public on Mar 21, 2024 |
| Title |
KO_Panc1_ABX_Rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Epithelioid Carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Epithelioid Carcinoma
cell line: Panc1
cell type: Pancreatic Cancer
genotype: WT
treatment: Nab_Paclitaxel
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using the QIAmp DNA blood Cell Maxi Kit (Qiagen) according to the manufacturer’s protocol.
The sgRNA sequences were amplified using Taq polymerase (Takara Bio, Inc.) and adapted for sequencing.
The desired DNA product was purified with 6% TBE gel (Invitrogen) and subjected to massive parallel amplicon sequencing carried out by an Illumina sequencer (NextSeq550 run with a 75Hi kit; 75bp single-end, one 8bp index).
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
NextSeq 550 |
| |
|
| Data processing |
The sgRNA read count and hits calling were analyzed by the MAGeCK v0.5.7 algorithm.
Assembly: hg38
Supplementary files format and content: Text file of sgRNA score in RRA
Library strategy: CRISPR
|
| |
|
| Submission date |
Mar 21, 2024 |
| Last update date |
Mar 21, 2024 |
| Contact name |
PRIYA MONDAL |
| E-mail(s) |
priya.mondal@nih.gov
|
| Phone |
(240) 630-9468
|
| Organization name |
NCI
|
| Street address |
37 Convent Dr.
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL21697 |
| Series (1) |
| GSE262149 |
A whole-genome CRISPR screen identifies the spindle accessory checkpoint as a locus of nab-paclitaxel resistance in pancreatic cancer cells. |
|
| Relations |
| BioSample |
SAMN40567610 |
| SRA |
SRX24014882 |
