|
| Status |
Public on May 01, 2024 |
| Title |
GFWT4 colon |
| Sample type |
RNA |
| |
|
| Source name |
Germ Free Colon
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Colon
gender: male
stran: Germ-free C57BL/6J WT
treatment: DSS
time: D7
|
| Treatment protocol |
To induce colitis, mice were administered drinking water supplemented with 2% (wt./vol.) dextran sulfate sodium (DSS; MP Biomedicals, LLC, Aurora, OH, USA) for 7 d and were then allowed to recover by drinking unsupplemented water for the next 5 d
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from total tissue of colon by using the column-based RNeasy® Mini Kit (Qiagen). RNA was quantified using a NanoDrop-1000 spectrophotometer and RNA quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Samples with RNA integrity number (RIN) greater than 9 were selected for further analysis.
|
| Label |
Cy3
|
| Label protocol |
Labelling of total RNA (100ng) was performed with the One-Color Low Input Quick Amp Labelling kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Samples were hybridized to SurePrint G3 mouse GE 8x60K (G4858A-028005) arrays and scanned by G2565CA Scanner System (Agilent Technologies).
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting for 1x60k array slides (Scan Area 61x21.6 mm, Scan resolution 10um, Dye channel is set to Green and Green PMT is set to 100%).
|
| Description |
Gene expression at day 7 after induction of DSS colitis
GFWT04
|
| Data processing |
Image analyses and raw signal intensities were quantified by Feature Extraction Software v10.7.3.1 (Agilent Technologies) according to the GE1_107_Sep09 protocol.
|
| |
|
| Submission date |
Mar 27, 2024 |
| Last update date |
May 01, 2024 |
| Contact name |
Phuong Hang PHAM |
| E-mail(s) |
hp.pham@pareanbiotech.fr
|
| Organization name |
Parean Biotechnologies
|
| Department |
Data science
|
| Lab |
Bat B, Lab 3
|
| Street address |
7 allée Métis
|
| City |
Saint-Malo |
| ZIP/Postal code |
35400 |
| Country |
France |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE262585 |
Dissecting the respective role of microbiota and host genetics in the susceptibility of Card9-/- mice to colitis |
|
