|
| Status |
Public on Apr 24, 2024 |
| Title |
Dsquame, Forehead, Volunteer 9 |
| Sample type |
SRA |
| |
|
| Source name |
normal epidermal tissue
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: normal epidermal tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
All tapes collected from a single site were pooled into a single tube and underwent enzymatic digestion in a chaotropic buffer to extract genomic material from the adhesive. Nucleic acids were pricipatated and isolated using magnetic beads, automated on a KingFisher Apex instrument.
RNA libraries for RNA-seq were prepared using NEBNext Low Input RNA Library Prep Kit following manufacturer's protocol.
Poly A Enrichment RNA-seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 2000 |
| |
|
| Data processing |
Samples underwent demultiplexing and were converted to FASTQ files using BCL Convert version 2.4.0
Samples underwent splice aware mapping and alignment to human reference genome (hg38 No Alts, with decoys) using Dragen RNA version 4.0.4.
Dragen RNA version 4.0.4. was used to generate mapping metrics files.
Assembly: GRCh38
Supplementary files format and content: tab-delimited text files include TPM values for each Sample
|
| |
|
| Submission date |
Mar 29, 2024 |
| Last update date |
Apr 24, 2024 |
| Contact name |
Erica Montano |
| E-mail(s) |
jostojic@dermtech.com, erica.montano@dermtech.com
|
| Organization name |
DermTech
|
| Street address |
12340 El Camino Real
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92130 |
| Country |
USA |
| |
|
| Platform ID |
GPL30173 |
| Series (1) |
| GSE262827 |
Comparison of Tape-Stripping Platforms as Non-Invasive Solutions for Skin Biomarker Discovery |
|
| Relations |
| BioSample |
SAMN40658096 |
| SRA |
SRX24105165 |
