|
| Status |
Public on Apr 03, 2024 |
| Title |
N0E0(32.12) |
| Sample type |
genomic |
| |
|
| Source name |
eihsc, NF1 deficient; EED deficient, origin 32.12
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: N0E0(32.12)
|
| Treatment protocol |
No treatment was performed after cell line generation.
|
| Growth protocol |
Cell lines were maintained and passaged at 37°C with 5% CO2 in DMEM high glucose media supplemented with 10% FBS and 1% penicillin/streptomycin.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted and purified from samples using Thermo Scientific GeneJET Genomic DNA Purification Kit according to standard instructions
|
| Label |
biotin
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina MethylationEPIC v2.0 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using Illumina iScan using standard recommended Illumina scanner setting
|
| Description |
11-N32E12A
|
| Data processing |
GenomeStudio, minfi
|
| |
|
| Submission date |
Apr 03, 2024 |
| Last update date |
Apr 03, 2024 |
| Contact name |
David Largaespada |
| Organization name |
University of Minnesota
|
| Department |
Department of Pediatrics
|
| Lab |
Largaespada Lab
|
| Street address |
515 Delaware St SE
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL21145 |
| Series (1) |
| GSE263127 |
Multiomic Analyses Reveal New Targets of Polycomb Repressor Complex 2 in Schwann Lineage Cells and Malignant Peripheral Nerve Sheath Tumors - Methylation |
|
