|
| Status |
Public on Jan 01, 2014 |
| Title |
CML_3647_I2 |
| Sample type |
RNA |
| |
|
| Source name |
CML Patient: Treated
|
| Organism |
Homo sapiens |
| Characteristics |
source: Bone Marrow
tissue type: tumor, treated
age: 60
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from purified cell populations using the RNeasy Mini Kit (Qiagen, Valencia, CA,USA) according to the manufacturer’s instructions. The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent,
Palo Alto, CA, USA).
|
| Label |
biotin
|
| Label protocol |
Labeling was performed according to protocols from Affymetrix. Briefly, 5000 ng of total RNA was amplified and labeled. These samples were fragmented and combined with array hybridization controls (Affymetrix, Santa Clara, CA, USA) in hybridization buffer. 11 µg of cRNA target were then hybridized with the GeneChip HG_U133 Plus 2.0 array (Affymetrix)
|
| |
|
| Hybridization protocol |
Hybridizations were performed according to protocols from Affymetrix.
Labeled RNA was hybridized to Human Genome U133 plus 2.0 microarray (Affymetrix).
|
| Scan protocol |
Washing and scanning were performed using Fluidics Station 400 and
GeneChip Scanner (Affymetrix Inc.).
|
| Data processing |
Expression value for each probe set was calculated using RMAExpress program that uses RMA (robust multi-array average) algorithm.
|
| |
|
| Submission date |
Oct 18, 2011 |
| Last update date |
Jan 01, 2014 |
| Contact name |
ROCIO BENITO |
| E-mail(s) |
beniroc@usal.es
|
| Organization name |
centro investigacion del cancer
|
| Lab |
12
|
| Street address |
Campus Unamuno s/n
|
| City |
Salamanca |
| ZIP/Postal code |
37007 |
| Country |
Spain |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE33075 |
Imatinib therapy of chronic myeloid leukemia restores the expression levels of key genes for DNA damage and cell cycle progression |
|
