|
Status |
Public on Nov 01, 2011 |
Title |
Sox3 over-expression in neural progenitor cells, biol repl 1 |
Sample type |
RNA |
|
|
Source name |
Neural progenitor cells
|
Organism |
Mus musculus |
Characteristics |
cell type: ES cell derived neural progenitor cells genotype: Sox1-GFP and Nestin-Sox3
|
Treatment protocol |
Cells were trypsinized and FACS sorted at day 4.
|
Growth protocol |
Sox1-GFP or Sox1-GFP/Nestin-Sox3 positive mouse ES-cells were differentiated for 4 days with B27 and N2 supplement (GIBCO) as monolayers on gelatinized cellbinding surface (Corning) with 0.5uM retinoic acid (all-trans, Sigma) at day 0 and day 2.
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen mRNA preparation kit
|
Label |
biotin
|
Label protocol |
Standard Affymetrix protocol.
|
|
|
Hybridization protocol |
Standard Affymetrix protocol.
|
Scan protocol |
Standard Affymetrix protocol.
|
Description |
Sox3 over-expression MB4_+Sox3_Sox1+NPC.CEL
|
Data processing |
The data was processed with RMA using Affymetrix Power Tools and a custom CDF (Brainarray version 11.0.1, RefSeq).
|
|
|
Submission date |
Oct 18, 2011 |
Last update date |
Nov 01, 2011 |
Contact name |
Rickard Sandberg |
E-mail(s) |
Rickard.Sandberg@ki.se
|
Organization name |
Karolinska Institutet
|
Department |
Department of Cell and Molecular Biology
|
Street address |
Berzelius vag 35
|
City |
Stockholm |
ZIP/Postal code |
17177 |
Country |
Sweden |
|
|
Platform ID |
GPL14757 |
Series (2) |
GSE33024 |
Sequentially acting Sox transcription factors in neural lineage development |
GSE33061 |
Sequentially acting Sox transcription factors in neural lineage development [microarray] |
|