|
| Status |
Public on Oct 20, 2011 |
| Title |
TNC knockout biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
E15.5 lumbar spinal cord tissue
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lumbar spinal cord
strain: SV129
age: E15.5
genotype: TNC -/-
|
| Treatment protocol |
not applicable
|
| Growth protocol |
mouse lumbar dissected from day E15.5 mouse embryos
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Qiagen
|
| Label |
biotin
|
| Label protocol |
As per manufacturers (WT expression kit Ambion, WT terminsal labelling kit Affymetrix)
|
| |
|
| Hybridization protocol |
45°C 16hrs 60rpm according to Affymetrix protocol, Fluidics Station Program FS45_00007
|
| Scan protocol |
Array Scanner 30007G, AGCC
|
| Data processing |
The image data were analyzed with Affymetrix® GeneChip® Command Console® Software (AGCC) (Affymetrix, USA), Normalization was performed using the RMA algorithm.
|
| |
|
| Submission date |
Oct 19, 2011 |
| Last update date |
Jun 06, 2013 |
| Contact name |
Bernd Denecke |
| E-mail(s) |
bernd.denecke@rwth-aachen.de
|
| Phone |
+49 241 8089918
|
| Organization name |
RWTH Aachen University
|
| Department |
IZKF Aachen
|
| Street address |
Pauwelsstrasse 30
|
| City |
Aachen |
| State/province |
NRW |
| ZIP/Postal code |
52074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE33091 |
Tenascin-C modifies expression levels and territories of key patterning genes during spinal cord astrocyte specification [mus musculus] |
|
