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| Status |
Public on Apr 16, 2024 |
| Title |
Spleen monocytes CD115+ PH70% 24 h 3 |
| Sample type |
SRA |
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| Source name |
Spleen
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| Organism |
Mus musculus |
| Characteristics |
tissue: Spleen
cell type: monocytes CD115+
treatment: spleen intact, 70% liver resection
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| Treatment protocol |
In this work, the effect of splenectomy on liver regeneration was studied. For this purpose in the animals were reproduced splenectomy, and after 7 days 70% liver resection was performed. Animals in which the spleen was intact and only 70% liver resection was performed served as a comparison group. Also, the control group included in whom only the spleen was removed. Peripheral blood and spleen monocytes from intact animals (spleen and liver intact) and from animals in which the liver was resected (spleen intact) were isolated for transcriptome analysis.
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| Extracted molecule |
total RNA |
| Extraction protocol |
The mouse mononuclear cell fraction was obtained from peripheral blood and spleen by gradient centrifugation. After that, the cells were subjected to immunomagnetic sorting on a manual MidiMACS™ Separator using LS Columns (Miltenyi Biotec, Bergisch Gladbach,Germany) and using Anti-CD115 MicroBeads magnetic microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) in accordance with the manufacturer's recommendations. For each sample, monocytes were obtained from 5 mice.
DNBSEQ Eukaryotic Strand-specific mRNA library
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
DNBSEQ-G400 |
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| Data processing |
Data filtering includes removing adaptor sequences, contamination and low-quality reads from raw reads
nf-core/rnaseq pipeline with STAR aligner
Assembly: GRCm39
Supplementary files format and content: comma delimeted text files include RPKM values for each Sample
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| Submission date |
Apr 11, 2024 |
| Last update date |
Apr 16, 2024 |
| Contact name |
Evgeny Karpulevich |
| E-mail(s) |
karpulevich@ispras.ru
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| Organization name |
ISP RAS
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| Department |
Information Systems
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| Street address |
Alexandra Solzhenitsina
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| City |
Moscow |
| ZIP/Postal code |
109004 |
| Country |
Russia |
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| Platform ID |
GPL28457 |
| Series (1) |
| GSE263764 |
Effect of splenectomy on liver regeneration after 70% resection in a mouse model. |
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| Relations |
| BioSample |
SAMN40931545 |
| SRA |
SRX24225385 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM8198165_SN07.csv.gz |
257.2 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
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