|
| Status |
Public on Dec 15, 2011 |
| Title |
undifferentiated cKit+ SSEA+ ESC rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
V6.5 ESC
|
| Organism |
Mus musculus |
| Characteristics |
cell type: undifferentiated cKit+ SSEA+ ESC
genetic background: 129xBL6 F1
|
| Treatment protocol |
Cells were dissociated using 0.05% trypsin, subjected to FACS sorting
|
| Growth protocol |
Cells were grown for three to six days under 37C, 5% CO2 and harvest via FACS at room temperature.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were collected in RLT Buffer and RNA isolated with the Qiagen Rneasy micro Kit.
|
| Label |
biotin
|
| Label protocol |
RNA was prepared usng Nu-GEN Ovation Pico WTA System and FL-Ovation cDNA Biotin Module V2.
|
| |
|
| Hybridization protocol |
Hybridization to Afftmetrix mouse 430 v2 chips and was performed as recommended by manufacturer. Arrays were washed and stained with streptavidin phycoerythrin in Affy Fluidics Station 450using Affymetrix GeneChip protocol.
|
| Scan protocol |
Chips were scanned using an Affymetrix GeneChip Scanner 30007G using manufacturer's recommended protocol.
|
| Description |
Gene expression of undifferentiated ESCs
|
| Data processing |
The data were analyzed with MicroArray Suite Version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Oct 20, 2011 |
| Last update date |
Dec 15, 2011 |
| Contact name |
John Vincent |
| E-mail(s) |
jvincent@ucla.edu
|
| Organization name |
UCLA
|
| Department |
MCDB
|
| Street address |
621 Charles E Young Dr. S
|
| City |
Los Angeles |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE33121 |
Expression data from ESC and in vitro derived somatic cells and germ cells |
|
