|
| Status |
Public on Dec 31, 2014 |
| Title |
Stem Cell |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Stem Cell
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: tumor stem cell obtained from human renal tumor xenografted into nude mice
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated from human renal cancer and from spheres using DNeasy MiniKit (Qiagen, France) according to the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
The digested DNAs were labeled with random primers using Agilent Genomic DNA Labeling Kit Plus for 2 hours at 37°C, according to the manufacturer’s instructions
|
| |
|
| Channel 2 |
| Source name |
Universal Reference HAPMAP (Promega, USA, Madison)
|
| Organism |
Homo sapiens |
| Characteristics |
reference: Human Universal Reference HAPMAP (Promega, USA, Madison)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated from human renal cancer and from spheres using DNeasy MiniKit (Qiagen, France) according to the manufacturer’s protocol.
|
| Label |
Cy5
|
| Label protocol |
The digested DNAs were labeled with random primers using Agilent Genomic DNA Labeling Kit Plus for 2 hours at 37°C, according to the manufacturer’s instructions
|
| |
|
| |
| Hybridization protocol |
The digested DNAs were labeled with random primers using Agilent Genomic DNA Labeling Kit Plus for 2 hours at 37°C, according to the manufacturer’s instructions
|
| Scan protocol |
were scanned with an Agilent DNA Microarray Scanner (G2565BA)
|
| Data processing |
Log ratios were determined with Agilent Feature Extraction software (v 9.1.3.1)
|
| |
|
| Submission date |
Oct 21, 2011 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Anne Janin |
| E-mail(s) |
jean-paul.feugeas@sls.aphp.fr
|
| Phone |
+33142494012
|
| Organization name |
University Paris 7, INSERM, APHP
|
| Street address |
1 Av C Vellefaux
|
| City |
Paris |
| ZIP/Postal code |
75010 |
| Country |
France |
| |
|
| Platform ID |
GPL10123 |
| Series (1) |
| GSE32414 |
Hypoxia-induced stem-like cancer cells resistance to VEGFR inhibition |
|
