|
| Status |
Public on Apr 16, 2024 |
| Title |
dpf47_Ov_mt_3 |
| Sample type |
SRA |
| |
|
| Source name |
Ovary
|
| Organism |
Danio rerio |
| Characteristics |
tissue: Ovary
age: dpf 47
genotype: mutant
|
| Growth protocol |
WT AB strain and mutant zebrafish lines were housed in the zebrafish core facility with a 14-hour(h) light and 10h dark photoperiod, lights on at 8:30am, lights off at 10:30pm; at water temperature of 28.5°C, pH of approximately 7.2, and salinity/conductivity ranging from 500 to 1,200µS in two automatically controlled standalone zebrafish rearing systems. Fish are fed to satiation three times daily with a commercial food.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Ovary tissues were homogenized immediately in RNAzol RT reagent and stored at -800C till purification. Total RNA was purified following the manufacturer’s protocol
cDNA was made for three replicates for each time point by genotype with the Tecan Ovation RNAseq kit for 47 and 61dpf samples. Libraries were then prepared with the Tecan Celero EZ DNA-Seq library kit for 47 and 61dpf samples.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Data were pseudoaligned with Kallisto.
Assembly: GRCz11
Supplementary files format and content: tab-delimited file that has raw counts for each sample.
|
| |
|
| Submission date |
Apr 16, 2024 |
| Last update date |
Apr 16, 2024 |
| Contact name |
Ciro Maurizio Amato |
| E-mail(s) |
amato.ciro27@gmail.com
|
| Phone |
2398257829
|
| Organization name |
National Institute of Environmental Health Sciences
|
| Department |
Reproductive and Developmental Biology Laboratory
|
| Lab |
Reproductive Developmental Biology
|
| Street address |
111 Tw Alexander Dr
|
| City |
Research Triangle Park |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL20828 |
| Series (1) |
| GSE264143 |
Adamts9 Is Required for Development of Primary Ovarian Follicles and Maintenance of Female Sex in Zebrafish |
|
| Relations |
| BioSample |
SAMN40985268 |
| SRA |
SRX24276186 |
