|
| Status |
Public on Oct 25, 2011 |
| Title |
Diseased plants rep2 |
| Sample type |
RNA |
| |
|
| Source name |
tomato leaves from inoculated plants with Phytophthora infestans
|
| Organism |
Solanum lycopersicum |
| Characteristics |
cultivar: Money Maker
tissue type: leaves
timepoint: 12 hours post inoculation
|
| Growth protocol |
Tomato plants cv. Money Maker of 5 weeks old were grown under greenhouse conditions. The plants were inoculated with 105 sporangia ml-1 of Phytophthora infestans (Mont.) de Bary. The whole plants were kept at 100% RH in the dark at 18°C and 12 hours post inoculation the leaves from both healthy non-inoculated and pathogen inoculated plants were used to extract the RNA.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolation was performed using NucleoSpinTM 8 RNA Isolation Kit (Machery-Nagel GmbH & Co. KG, Düren, Germany) according to the manufacturer's instruction.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was labeled using a biotinylated nucleotide analog/ribonucleotide mix using GeneChip IVT Labeling Kit (Affymetrix, Inc., Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cRNA were hybridized for 16 hr on GeneChip Tomato Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip scanner 3000 integrated with Affymetrix® Microarray Suitesoftware.
|
| Data processing |
The microarray data normalization and background correction was performed using Guanine Cytosine Robust Multi-Array Analysis (GCRMA)
|
| |
|
| Submission date |
Oct 24, 2011 |
| Last update date |
Oct 25, 2011 |
| Contact name |
Muna Sultan |
| E-mail(s) |
muna_soltan@yahoo.com
|
| Organization name |
university of Bonn
|
| Street address |
nussalle 9
|
| City |
Bonn |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4741 |
| Series (1) |
| GSE33177 |
Microarray analysis of gene expression induced by Phytophthora infestans in tomato leaves |
|
