|
| Status |
Public on Apr 18, 2024 |
| Title |
AGS HP+, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
WT H. pylori-infected AGS cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: AGS cells
treatment: H. pylori-infected
|
| Treatment protocol |
AGS cells were infected with WT H. pylori at a multiplicity of infection (MOI) of 100 for 6 h.
|
| Growth protocol |
AGS cells were cultured in complete RPMI 1640 medium supplemented with 10% FBS in a humidified environment containing 5% CO2 at 37 °C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from WT H. pylori-infected AGS cells
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Apr 17, 2024 |
| Last update date |
Apr 18, 2024 |
| Contact name |
yuan zhuang |
| E-mail(s) |
yuanzhuang1983@yahoo.com
|
| Phone |
136 3795 4752
|
| Organization name |
Third Military Medical University
|
| Street address |
Gaotanyan Street
|
| City |
Chongqing |
| ZIP/Postal code |
400038 |
| Country |
China |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE264263 |
Expression data from AGS cells with Helicobacter pylori infection |
|
