|
Status |
Public on Jun 01, 2024 |
Title |
RNAseq_PDAC_8661_DMSO_r2 |
Sample type |
SRA |
|
|
Source name |
8661
|
Organism |
Mus musculus |
Characteristics |
cell line: 8661 cell type: pancreatic ductal adenocarcinoma genotype: Ptf1a-Cre +, LSL-Kras G12D mut/wt, klassisch treatment: DMSO 24 h
|
Treatment protocol |
Cells were treated with 1 µM CB-6644 or DMSO for 24 h.
|
Growth protocol |
8661, 9091, 9172, 24033 and SB1557 cells were grown in DMEM suplemented with 10 % FCS and 1% Penicillin/Streptomycin.
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA was extracted using miRNeasy kit (Qiagen) NEBNext rRNA Depletion v2 followed by NEBNext Ultra II RNA Library Prep kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
NextSeq 2000 |
|
|
Description |
TOC_PDAC_8661_CB6644.tsv DGE_PDAC_8661_CB6644.tsv
|
Data processing |
STAR alignment to the mm39 reference sequence gene-level reads (Ensembl version 111) were counted using GenomicAlignments R package (version 1.38.2) Differential gene expression analysis using edgeR (version 4.0.16) Assembly: mm39 Supplementary files format and content: .tsv file containing the results of differential gene expression analysis for each cell line upon CB-6644 treatment.
|
|
|
Submission date |
Apr 18, 2024 |
Last update date |
Jun 01, 2024 |
Contact name |
Elmar Wolf |
E-mail(s) |
elmar.wolf@biozentrum.uni-wuerzburg.de
|
Organization name |
Biocenter of the University of Würzburg
|
Department |
Department of Molecular Biology and Biochemistry
|
Lab |
Cancer Systems Biology Lab
|
Street address |
Am Hubland
|
City |
Würzburg |
State/province |
Bavaria |
ZIP/Postal code |
97074 |
Country |
Germany |
|
|
Platform ID |
GPL30172 |
Series (2) |
GSE216095 |
Targeting MYC effector functions in pancreatic cancer by inhibiting the ATPase RUVBL1/2 |
GSE264280 |
Targeting MYC effector functions in pancreatic cancer by inhibiting the ATPase RUVBL1/2 (RNA-seq) |
|
Relations |
BioSample |
SAMN41006721 |
SRA |
SRX24299498 |