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| Status |
Public on Jun 18, 2024 |
| Title |
DMSO w1118,RNA-seq,Rep3 |
| Sample type |
SRA |
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| Source name |
3rd instar whole larvae
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| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: 3rd instar whole larvae
genotype: w1118
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by standard protocol with Trizol; 1ug RNA was used for librariy construction
RNA-seq library was prepared by using Illumina TruSeq Stranded Total RNA kit, CUT&Run library was prepared by using illumina TruSeq ChIP library preparation kit
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
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| Description |
DMSO w1118 3
BTZ count table
BTZ_cts.txt
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| Data processing |
RNA-Star alignment to dm6 reference with default setting
Gathering counts with FeatureCount with default setting
DESeq2 to normalize counts and generate differential gene expression table with p.adj < 0.05 filter
CUT&Run reads went through nf-core/cutandrun pipeline for trimming, alignment and deduplication
MACS3 for peak calling and normalization
Assembly: dm6
Supplementary files format and content: tab delimited table, count table
Supplementary files format and content: bigwig file, normalized CUT&Run reads
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| Submission date |
Apr 18, 2024 |
| Last update date |
Jun 18, 2024 |
| Contact name |
Jun-yuan Ji |
| E-mail(s) |
ji@tulane.edu
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| Organization name |
Tulane University
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| Department |
Department of Biochemistry and Molecular Biology
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| Street address |
1700 Tulane Avenue
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| City |
New Orleans |
| State/province |
LA |
| ZIP/Postal code |
70112 |
| Country |
USA |
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| Platform ID |
GPL17275 |
| Series (1) |
| GSE264356 |
Wnt/Wingless signaling promotes lipid mobilization through signal-induced transcriptional repression |
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| Relations |
| BioSample |
SAMN41010373 |
| SRA |
SRX24304930 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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