|
| Status |
Public on May 05, 2024 |
| Title |
Macrophages, MCS_ZT14 |
| Sample type |
SRA |
| |
|
| Source name |
peritoneal macrophage
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
cell type: peritoneal macrophage
treatment: microcurrent-stimulation
time: ZT14
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was isolated from cells using TRIzol Reagent (nitrogen) and purified using SV Total RNA Isolation System (Promega) according to the manufacturer's instructions.
RNA libraries were prepared for sequencing using MGIEasy rRNA Depletion Kit and MGIEasy RNA Directional Library Prep Set according protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
DNBSEQ-G400 |
| |
|
| Data processing |
MGI DNBseq-G400 FAST was used to perform the amplicons deep sequencing following the standard operation protocol. The sequence format was 150bp pair read for all samples.
All sequencing reads were trimmed low-quality bases and adapters with Trimmomatic (v.0.38) .
Raw counts for each gene were estimated in each sample using RSEM version 1.3.0 and Bowtie 2. Calculation of the log fold-change (log FC) and p-value were performed using edgeR.
Assembly: mm10
Supplementary files format and content: tab-delimited text file. normalized CPM count matrix with GeneSymbol index.
|
| |
|
| Submission date |
Apr 25, 2024 |
| Last update date |
May 05, 2024 |
| Contact name |
Yuya Yoshida |
| E-mail(s) |
yoshida@phar.kyushu-u.ac.jp
|
| Phone |
0926426658
|
| Organization name |
Kyushu-university
|
| Street address |
東区馬出3-1-1 九州大学薬学部棟本館3F 薬剤学教室, 薬物動態学分野
|
| City |
福岡市 |
| State/province |
福岡県 |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL28457 |
| Series (1) |
| GSE265902 |
The effect of microcurrent-stimulation on mouse macrophages |
|
| Relations |
| BioSample |
SAMN41084177 |
| SRA |
SRX24371648 |
