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Status |
Public on Sep 01, 2012 |
Title |
TSC22D4 Knockdown Mouse Liver 5 + 6 (pool) |
Sample type |
RNA |
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Source name |
Liver, excized after 1 week of knockdown
|
Organism |
Mus musculus |
Characteristics |
strain: C57BI/6 genotype/variation: Adenovirus mediated shRNA knockdown of TSC22D4 tissue: Liver, excized after 1 week of knockdown
|
Treatment protocol |
Adenovirus mediated shRNA knockdown of TSC22D4 in the liver
|
Growth protocol |
Mice were fed ad libitum and sacrificed in the morning.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Mini Kit (Quiagen)
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
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|
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Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
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Scan protocol |
Affymetrix GeneArray Scanner3000
|
Description |
TSC_C
|
Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of Mouse430_2 -type from Affymetrix. A Custom CDF Version 12 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
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Submission date |
Oct 28, 2011 |
Last update date |
Sep 01, 2012 |
Contact name |
Carsten Sticht |
Organization name |
University Heidelberg
|
Department |
ZMF
|
Street address |
Theodor-Kutzer-Ufer
|
City |
Mannheim |
ZIP/Postal code |
68169 |
Country |
Germany |
|
|
Platform ID |
GPL9746 |
Series (1) |
GSE33324 |
Cachexia-inducible Transforming growth factor-beta1 stimulated Clone-22 D4 controls acute hepatic lipid homeostasis. |
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