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Sample GSM824085 Query DataSets for GSM824085
Status Public on Sep 01, 2012
Title TSC22D4 Knockdown Mouse Liver 5 + 6 (pool)
Sample type RNA
 
Source name Liver, excized after 1 week of knockdown
Organism Mus musculus
Characteristics strain: C57BI/6
genotype/variation: Adenovirus mediated shRNA knockdown of TSC22D4
tissue: Liver, excized after 1 week of knockdown
Treatment protocol Adenovirus mediated shRNA knockdown of TSC22D4 in the liver
Growth protocol Mice were fed ad libitum and sacrificed in the morning.
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit (Quiagen)
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description TSC_C
Data processing The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of Mouse430_2 -type from Affymetrix. A Custom CDF Version 12 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
 
Submission date Oct 28, 2011
Last update date Sep 01, 2012
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL9746
Series (1)
GSE33324 Cachexia-inducible Transforming growth factor-beta1 stimulated Clone-22 D4 controls acute hepatic lipid homeostasis.

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 7.25293
AFFX-BioB-M_at 8.646484
AFFX-BioB-3_at 7.861328
AFFX-BioC-5_at 9.207031
AFFX-BioC-3_at 9.460938
AFFX-BioDn-5_at 10.39258
AFFX-BioDn-3_at 11.62305
AFFX-CreX-5_at 13.10938
AFFX-CreX-3_at 13.41602
AFFX-DapX-5_at 3.199707
AFFX-DapX-M_at 3.174316
AFFX-DapX-3_at 3.105957
AFFX-LysX-5_at 3.096191
AFFX-LysX-M_at 3.262207
AFFX-LysX-3_at 3.138672
AFFX-PheX-5_at 3.177734
AFFX-PheX-M_at 3.053711
AFFX-PheX-3_at 4.155273
AFFX-ThrX-5_at 3.385254
AFFX-ThrX-M_at 3.255371

Total number of rows: 16395

Table truncated, full table size 292 Kbytes.




Supplementary file Size Download File type/resource
GSM824085_Jones_170610_TSC-C_Mouse430_2_.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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