|
| Status |
Public on May 31, 2024 |
| Title |
PerM, WT control |
| Sample type |
RNA |
| |
|
| Source name |
Peritoneal macrophages
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: WT
cell type: Peritoneal macrophages
treatment: control
|
| Treatment protocol |
Cells were treated with 1ug/ml rmCIRP for 24 h prior to to gene expression analysis
|
| Growth protocol |
PerM was cultured in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS), 2 mM glutamine, 100 IU/ml penicillin‒streptomycin, and 25 mM HEPES at 37 °C in 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with RNAspin Mini kit with DNase 1 treatment
|
| Label |
SYBR Green
|
| Label protocol |
PCR assay were performed using mouse circadian rhythms PCR array (Qiagen, Germantown, MD) following the Manufacture's instructions
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Control
SAMPLE 1
|
| Data processing |
The normalization and all the data analysis were performed according to the manufacturers instructions using their web-based software package: https://geneglobe.qiagen.com/us/analyze. Target gene signals normalized to house keeping gene ACTB
Fold Change worksheet reports test/control (i.e., rmCIRP/PBS) ratios.
|
| |
|
| Submission date |
May 05, 2024 |
| Last update date |
May 31, 2024 |
| Contact name |
Atsushi Murao |
| E-mail(s) |
amurao@northwell.edu
|
| Organization name |
The Feinstein Institutes for Medical Research
|
| Street address |
350 Community Dr
|
| City |
Manhasset |
| ZIP/Postal code |
11030 |
| Country |
USA |
| |
|
| Platform ID |
GPL34452 |
| Series (1) |
| GSE266607 |
Real-time quantitative PCR analysis of mouse peritoneal macrophages |
|
