|
Status |
Public on Nov 22, 2011 |
Title |
species: Corn - rep2 |
Sample type |
RNA |
|
|
Source name |
8##GRO:0005306## (species: Corn)
|
Organism |
Zea mays |
Characteristics |
age: 7d tissue: seedling
|
Growth protocol |
Growth Description: Seedlings were grown in the greenhouse for 7 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Hot Phenol/Guanidine Thiocyanate and RNeasy Clean up
|
Label |
biotin
|
Label protocol |
Affy-Eukaryotic Target Preparation
|
|
|
Hybridization protocol |
Affy- Eukaryotic Target Hybridization
|
Scan protocol |
Affy- Array Scanning
|
Description |
gro: 0005118##GRO:0005306## (Organism Part); GRO:0007060 (Development Stage); 7 days (Age);
|
Data processing |
The MAS5.0 Normalization is performed using the justMAS function in the simpleaffy package of Bioconductor (www.bioconductor.org). The target value is 500 for all cases. The code was developed using R2.4.1, Bioconductor 1.9, and simpleaffy 2.8.0. The CDF files were downloaded from the Bioconductor website at http://www.bioconductor.org/packages/release/AffymetrixChip.html. The normalization scripts are available from the PLEXdb team by request.
|
|
|
Submission date |
Nov 02, 2011 |
Last update date |
Nov 22, 2011 |
Contact name |
PLEXdb Curator, Sudhansu Dash |
Organization name |
Iowa State University
|
Department |
Virtual Reality Application Center
|
Lab |
PLEXdb
|
Street address |
1023 Crop Genomes Informatics Lab (Iowa State University)
|
City |
Ames |
State/province |
IA |
ZIP/Postal code |
50011 |
Country |
USA |
|
|
Platform ID |
GPL1340 |
Series (1) |
GSE33391 |
Cross-species Detection in Barley1 GeneChip Array |
|