|
| Status |
Public on Nov 05, 2011 |
| Title |
U87 EGFR EGF minus rep1 |
| Sample type |
RNA |
| |
|
| Source name |
U87 EGFR EGF minus rep1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U87MG
parental cell line: U87
treatment: none
|
| Treatment protocol |
U87 and U178 cells engineered to express EGFR were stimulated with or without 20 ng/mL of recombinant EGF for 3 h prior to sample collection.
|
| Growth protocol |
U87 and U178 cells engineered to express EGFR were serum starved for 24 h prior to stimulation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on human HG-U133A Affymetrix arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
|
| Description |
Gene expression data from U87-EGFR
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200. The comparison analysis was also performed to calculate the increased ratios (fold-change) after the stimulation of EGF in these cells.
|
| |
|
| Submission date |
Nov 03, 2011 |
| Last update date |
Nov 05, 2011 |
| Contact name |
Frank Furnari |
| E-mail(s) |
ffurnari@ucsd.edu
|
| Phone |
8585347819
|
| Organization name |
Ludwig Institute
|
| Lab |
Furnari
|
| Street address |
9500 Gilman Drive
|
| City |
LaJolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0660 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE33442 |
The target genes of EGFR activity in glioma cells |
|
