|
| Status |
Public on Jun 15, 2024 |
| Title |
CS10, YS, scRNA-seq |
| Sample type |
SRA |
| |
|
| Source name |
yolk sac
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: yolk sac
cell type: Hematopoietic cells
genotype: WT
treatment: in vivo
|
| Extracted molecule |
total RNA |
| Extraction protocol |
7-AAD– cells from one CS10 YS (in vivo) were sorted for scRNA-seq (10X Genomics). All generated CD45+ cells from cultured CS12b-YS were sorted for scRNA-seq (10X Genomics)
Libraries were prepared with Chromium Single cell 3' Reagent v2 Kits accoding to the manufacturer's instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
7-AAD– cells from one CS10 YS (in vivo)
|
| Data processing |
The Cell Ranger software pipeline (version 4.0.0) provided by 10xGenomics was used to demultiplex cellular barcodes, map reads to the human reference genome,and filter data for valid cell barcodes and unique molecular identifiers
Assembly: hg19
Supplementary files format and content: Matrix table with raw gene counts for every gene and every cell
|
| |
|
| Submission date |
May 22, 2024 |
| Last update date |
Jun 15, 2024 |
| Contact name |
Guoju You |
| E-mail(s) |
ygj19@mails.tsinghua.edu.cn
|
| Phone |
18801365214
|
| Organization name |
Tsinghua University
|
| Street address |
Shuangqing Road
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
| |
|
| Platform ID |
GPL20795 |
| Series (2) |
| GSE268142 |
Uncovering human yolk sac-derived innate lymphoid-biased multipotent progenitors prior to hematopoietic stem cell formation (scRNA-Seq) |
| GSE268143 |
Uncovering human yolk sac-derived innate lymphoid-biased multipotent progenitors prior to hematopoietic stem cell formation |
|
| Relations |
| BioSample |
SAMN41494604 |
| SRA |
SRX24655014 |
