|
| Status |
Public on May 29, 2024 |
| Title |
Cell_BJF_Trajectory_A_PDL_24 |
| Sample type |
genomic |
| |
|
| Source name |
BJ-CRL-2522
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: Male
cell line: BJ-CRL-2522
cell type: Fibroblast
|
| Growth protocol |
Cells were routinely cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS (REF: 10437-028), 1x Gibco MEM Non-Essential Amino Acids (REF: 11140-050), and 1x GlutaMax (REF: 35050079)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNeasy Blood and Tissue Kit (Qiagen 69504)
|
| Label |
Cy3 and Cy5
|
| Label protocol |
Standart Illumina
|
| |
|
| Hybridization protocol |
EZ-96 DNA Methylation-Lightning™ MagPrep (Zymo)
|
| Scan protocol |
iScan Illuminias settings
|
| Description |
WT24A
|
| Data processing |
SESAME
Beta Values
|
| |
|
| Submission date |
May 22, 2024 |
| Last update date |
May 29, 2024 |
| Contact name |
Morgan E. Levine |
| E-mail(s) |
mlevine@altoslabs.com
|
| Organization name |
Altos Labs
|
| Department |
Institute of Sciences San Diego
|
| Street address |
5510 Morehouse Dr
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL21145 |
| Series (1) |
| GSE268145 |
Longitudinal DNA methylation in serially passaged BJ Fibroblasts to investigate the latent aging of cells |
|
