|
| Status |
Public on May 24, 2024 |
| Title |
Whole blood Animal PA64 at baseline (2wks) before full length CyCMV/flu vaccination |
| Sample type |
SRA |
| |
|
| Source name |
Whole Blood PAXgene
|
| Organism |
Macaca fascicularis |
| Characteristics |
time point: Baseline
vaccination outcome: Non protected
Sex: Male
animall id: PA64
tissue: Whole Blood PAXgene
treatment: full length CyCMV/flu
|
| Treatment protocol |
Cynomolgus macaques received subcutanous adminstration of a prime and boost of either the 68-1 CyCMV/flu or full length 68-1 CyCMV/flu vaccines
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole blood was collected from cynomolgus macaques in PAXgene RNA tubes (PreAnaytiX) to manufacturers instrucruction. RNA was isolated using RNAdvance Blood Kit with the manufacter's instructions
Libraries were constructed using Illumina TruSeq Stranded mRNA HT kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
G007_Sacha01_2_PA64_B_BSLN
|
| Data processing |
Sequenced reads were trimmed with Trim galore using default parameters version 0.6.4
Globin and rRNA reads filtered out with bowtie2 version 2.4.2
Reads aligned and quantified using STAR v 2.7.5 to rhesus macaque genome version10 with ensembl annotation version 100
Assembly: rhesus macaque genome version10 with ensembl annotation version 100
Supplementary files format and content: count_matrix.txt
|
| |
|
| Submission date |
May 23, 2024 |
| Last update date |
May 24, 2024 |
| Contact name |
Michael Gale, Jr |
| E-mail(s) |
uw_galelab_geo@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Immunology
|
| Street address |
750 Republican St. E360, Box 358059
|
| City |
Seattle |
| State/province |
Washington |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL22523 |
| Series (1) |
| GSE268204 |
Cytomegalovirus vaccine vector-induced effector memory CD4+ T cells protect cynomolgus macaques from lethal aerosolized heterologous avian influenza |
|
| Relations |
| BioSample |
SAMN41510543 |
| SRA |
SRX24664412 |
