|
| Status |
Public on May 30, 2024 |
| Title |
1_204963470004_R01C01_Participant 1_Male_1 Baseline |
| Sample type |
genomic |
| |
|
| Source name |
DNA from Vastus Lateralis Muscle_1_204963470004_R01C01_Participant 1_Male_1 Baseline
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: Male
tissue: Skeletal Muscle_Vastus Lateralis
timepoint: Baseline
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted via the Qiagen DNA Easy Kit in accordance with manufacturers guidelines. The DNA was then bisulfite converted using the EZ DNA Methylation Kit, Zymo Research, CA, United States, as per manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Labels (Sample number_Beadchip Barcode_Beadchip position_Participant No_Sex_Condition)
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human MethylationEPIC Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using Infinium Methylation NXT setting
|
| Description |
Genomic DNA from Skeletal Muscle Biopsy_1_204963470004_R01C01_Participant 1_Male_1 Baseline
|
| Data processing |
Partek Genomic Suite V7 (Using MethylationEPIC_v-1-0_B4 manifest file)
Data is normalised via functional normalisation (with noob background correction)
|
| |
|
| Submission date |
May 23, 2024 |
| Last update date |
May 30, 2024 |
| Contact name |
Adam P Sharples |
| E-mail(s) |
a.p.sharples@googlemail.com
|
| Organization name |
Norwegian School of Sport Sciences
|
| Street address |
220 Sognsveien
|
| City |
Oslo |
| ZIP/Postal code |
0863 |
| Country |
Norway |
| |
|
| Platform ID |
GPL21145 |
| Series (1) |
| GSE268211 |
Human skeletal muscle possesses an epigenetic memory of high intensity interval training |
|
