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| Status |
Public on Jul 27, 2024 |
| Title |
S2 |
| Sample type |
SRA |
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| Source name |
Cerebral cortex
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| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Cerebral cortex
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from each sample using the RNA mini kit ( Qiagen, Germany),1μg of total RNA was used for library construction
Sequencing libraries were generated using VAHTSTM Total RNA-seq (H/M/R) Library Prep Kit for Illumina®
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Description |
S2
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| Data processing |
the commercial service of Genergy Biotechnology Co. Ltd. (Shanghai, China)
For raw pair-end reads, adapters and poor quality bases were trimmed off using Skewer version 0.2.2
The clean reads were aligned to the genome reference genome downloaded from the Ensembl website ( http://ensemblgenomes.org/) using STAR version 2.5.3a and comprehensive gene annotation GTF file from Ensembl website
The mapped reads of each sample were then assembled into transcripts based on reference guided assembly strategy using StringTie version 1.3.1
Assembly: rn6
Supplementary files format and content: tab-delimited text file includes raw counts for each Sample
Supplementary files format and content: tab-delimited text file includes RPKM for each Sample
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| Submission date |
May 29, 2024 |
| Last update date |
Jul 27, 2024 |
| Contact name |
Nasir ABDUL |
| E-mail(s) |
anasirqau@gmail.com
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| Organization name |
Zhengzhou University
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| Department |
The Second Clinical Medical School of Zhengzhou University
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| Street address |
Jingba Road
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| City |
Zheng Zhou |
| ZIP/Postal code |
450003 |
| Country |
China |
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| Platform ID |
GPL25947 |
| Series (1) |
| GSE268634 |
Effect of dexmedetomidine on ncRNA and mRNA profiles of cerebral ischemia-reperfusion injury in transient middle cerebral artery occlusion rats model |
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| Relations |
| BioSample |
SAMN41593999 |
| SRA |
SRX24744889 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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