|
| Status |
Public on Nov 09, 2011 |
| Title |
hen1 mutant polyA |
| Sample type |
SRA |
| |
|
| Source name |
testis
|
| Organism |
Danio rerio |
| Characteristics |
strain: TL
genotype/variation: Hen1 mutant
tissue: testis
small rna library prep method: polyA tailing
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Small RNAs in the size range of 19-31 bases were excised from a denaturing gel. RNA was poly(A)-tailed using poly(A) polymerase followed by ligation of a RNA adaptor to the 5'-phosphate of the small RNAs. First-strand cDNA synthesis was performed using an oligo(dT)-linker primer and M-MLV-RNase H- reverse transcriptase. After amplification the cDNA was sent for sequencing on an Illumina/Solexa platform.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
VASAGFPHEN1minusMALE
Small RNA sequence data
Processed data file: VASAGFPHEN1minusMALE.processed.txt
barcode: first 4 bases
|
| Data processing |
3' adapter sequences were trimmed and inserts longer than 18 nt were mapped to the zebrafish genome (Zv8)
|
| |
|
| Submission date |
Nov 09, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Eugene Berezikov |
| E-mail(s) |
e.berezikov@umcg.nl
|
| Phone |
+31 50 361 73 00
|
| Organization name |
University Medical Center Groningen
|
| Department |
European Research Institute for the Biology of Ageing
|
| Street address |
Antonius Deusinglaan 1
|
| City |
Groningen |
| ZIP/Postal code |
9713AV |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL9319 |
| Series (1) |
|
| Relations |
| SRA |
SRX105295 |
| BioSample |
SAMN00752521 |
