|
Status |
Public on Nov 09, 2011 |
Title |
hen1 mutant polyA |
Sample type |
SRA |
|
|
Source name |
testis
|
Organism |
Danio rerio |
Characteristics |
strain: TL genotype/variation: Hen1 mutant tissue: testis small rna library prep method: polyA tailing
|
Extracted molecule |
total RNA |
Extraction protocol |
Small RNAs in the size range of 19-31 bases were excised from a denaturing gel. RNA was poly(A)-tailed using poly(A) polymerase followed by ligation of a RNA adaptor to the 5'-phosphate of the small RNAs. First-strand cDNA synthesis was performed using an oligo(dT)-linker primer and M-MLV-RNase H- reverse transcriptase. After amplification the cDNA was sent for sequencing on an Illumina/Solexa platform.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
size fractionation |
Instrument model |
Illumina Genome Analyzer II |
|
|
Description |
VASAGFPHEN1minusMALE Small RNA sequence data Processed data file: VASAGFPHEN1minusMALE.processed.txt barcode: first 4 bases
|
Data processing |
3' adapter sequences were trimmed and inserts longer than 18 nt were mapped to the zebrafish genome (Zv8)
|
|
|
Submission date |
Nov 09, 2011 |
Last update date |
May 15, 2019 |
Contact name |
Eugene Berezikov |
E-mail(s) |
e.berezikov@umcg.nl
|
Phone |
+31 50 361 73 00
|
Organization name |
University Medical Center Groningen
|
Department |
European Research Institute for the Biology of Ageing
|
Street address |
Antonius Deusinglaan 1
|
City |
Groningen |
ZIP/Postal code |
9713AV |
Country |
Netherlands |
|
|
Platform ID |
GPL9319 |
Series (1) |
|
Relations |
SRA |
SRX105295 |
BioSample |
SAMN00752521 |