|
| Status |
Public on Jun 13, 2024 |
| Title |
Larv deltaWT K27me3 Repli2 |
| Sample type |
SRA |
| |
|
| Source name |
larval imaginal leg discs
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: larval imaginal leg discs
genotype: Wild type
|
| Growth protocol |
Drosophila Melanogaste flies grown at 20°C on standard medium
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cut&Run experiments were performed as described by Kami Ahmad in protocilas.io (https://dx.doi.org/10.17504/protocols.io.umfeu3n)
Libraries were preapred according the protocol of the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Libraries sequencing quality were analyzed using fastQC software.
Alignment of paired-end reads was done using bowtie2 to the dm6 reference genome.
Alignments with MAPQ smaller than 30 were discarded. Duplicated paired-end reads were discarded using sambamba.
For data visualization, bigWig files were produced using bamCoverage software (deeptools), ignoring duplicate reads and applying RPKM normalization.
Assembly: dm6
Supplementary files format and content: bigwigs
|
| |
|
| Submission date |
Jun 10, 2024 |
| Last update date |
Jun 13, 2024 |
| Contact name |
Bernd Schuettengruber |
| E-mail(s) |
bernd.schuttengruber@igh.cnrs.fr
|
| Organization name |
Institute de Génétique Humaine
|
| Lab |
Cavalli
|
| Street address |
141, rue de la cardonille
|
| City |
Montpellier |
| ZIP/Postal code |
34396 |
| Country |
France |
| |
|
| Platform ID |
GPL25244 |
| Series (1) |
| GSE247377 |
A PRE loop at the dac locus acts as a topological chromatin structure that restricts and specifies enhancer promoter communication |
|
| Relations |
| BioSample |
SAMN41780615 |
| SRA |
SRX24866229 |
