|
| Status |
Public on Jun 20, 2024 |
| Title |
3-17T_R1:Escherichia coli str. BL21:AMPLICON |
| Sample type |
SRA |
| |
|
| Source name |
Escherichia coli str. BL21
|
| Organism |
Escherichia coli BL21 |
| Characteristics |
strain: BL21
cell type: Escherichia coli str. BL21
|
| Treatment protocol |
Plasmids for electro-transferring Taqtth and sgRNA expression
|
| Growth protocol |
E. coli BL21 cells were grown in LB medium at 37°C with shaking at 200 rpm until the OD600 reached 0.6.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen) according to the manufacturer's instructions.
The amplicon library was prepared by PCR amplification of the target region using specific primers. The PCR products were purified using AMPure XP beads (Beckman Coulter) and quantified using Qubit (Thermo Fisher Scientific). Libraries were constructed using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions.
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Illumina software used for basecalling.
Assembly: E. coli BL21 genome, NCBI assembly GCA_000001635.1.
Supplementary files format and content: The processed data files are provided in VCF format, containing variant calls for the sequenced regions.
Library strategy: Amplicon sequencing
|
| |
|
| Submission date |
Jun 11, 2024 |
| Last update date |
Jun 20, 2024 |
| Contact name |
Shu Xu |
| E-mail(s) |
shuxu@cpu.edu.cn
|
| Organization name |
China Pharmaceutical University
|
| Street address |
China Pharmaceutical University
|
| City |
Nanjing |
| State/province |
Jiangsu Province |
| ZIP/Postal code |
210009 |
| Country |
China |
| |
|
| Platform ID |
GPL34578 |
| Series (1) |
| GSE269593 |
Specific silencing of pathogenic mRNA by a novel compact RNA-targeting tool TaqTth-hpRNA [Amplicon sequencing] |
|
| Relations |
| BioSample |
SAMN41763311 |
| SRA |
SRX24850389 |
