|
| Status |
Public on Jun 20, 2024 |
| Title |
HMV-II cells shNC EZH2-ChIP |
| Sample type |
SRA |
| |
|
| Source name |
vaginal
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: vaginal
cell line: HMV-II
cell type: melanoma
chip antibody: EZH2 (CST, #5246)
|
| Growth protocol |
HMV-II cells were cultured in F10 containing 10% fetal bovine serum and 1% penicillin-streptomycin at 37°C with 5% CO2.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ChIP assays were performed using the SimpleChIP Enzymatic Chromatin IP Kit (9003, Cell Signaling Technology) following the manufacturer's instructions.
The library was constructed by Novogene Corporation (Beijing, China). Subsequently, pair-end sequencing of sample was performed on Illumina platform (Illumina, CA, USA). Library quality was assessed on the Agilent Bioanalyzer 2100 system.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Raw reads were mapped to the human genome (hg19) using BWA mem (v 0.7.12), and fragment size estimated with MACS2 [68]. Peaks were annotated with ChIPseeker and motifs identified with Homer. A q-value threshold of 0.05 was applied. Validation was performed with ChIP-qPCR.
Assembly: hg19
Supplementary files format and content: bigwig for each sample
|
| |
|
| Submission date |
Jun 13, 2024 |
| Last update date |
Jun 20, 2024 |
| Contact name |
Haizhen Du |
| E-mail(s) |
2111110621@stu.pku.edu.cn
|
| Phone |
17839914360
|
| Organization name |
Peking University Cancer Hospital and Institute
|
| Street address |
No.52 Fucheng Road, Haidian District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100142 |
| Country |
China |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE269788 |
EZH2 protects mucosal melanoma from ferroptosis via the KLF14-SLC7A11 signaling pathway (ChIP-Seq) |
|
| Relations |
| BioSample |
SAMN41818561 |
| SRA |
SRX24912563 |
