|
| Status |
Public on Jun 17, 2024 |
| Title |
Pro-T_Cas9_EV+EV+gLuc_R3 |
| Sample type |
SRA |
| |
|
| Source name |
Pro-T cell line
|
| Organism |
Mus musculus |
| Characteristics |
cell line: Pro-T cell line
treatment: EV+EV+gLuc
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using a Promega Maxwell, using the Maxwell RSC simplyRNA Cells Kit
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq X |
| |
|
| Data processing |
cleaning with fastq-mcf
quality control with FastQC
mapping with HISAT2, further processing with SAMTools
counting abundances with htseq-count
Assembly: Mus Musculus (mm10)
Supplementary files format and content: tab-delimited text file includes raw count values for each gene
|
| |
|
| Submission date |
Jun 17, 2024 |
| Last update date |
Jun 17, 2024 |
| Contact name |
Sofie Demeyer |
| E-mail(s) |
sofie.demeyer@kuleuven.be
|
| Phone |
+32485895495
|
| Organization name |
VIB-KU Leuven Center for Cancer Biology
|
| Street address |
Herestraat 49, Campus Gasthuisberg, O&N 4, 9e verd, bus 912
|
| City |
Leuven |
| ZIP/Postal code |
3000 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL34328 |
| Series (1) |
| GSE241640 |
TLX3 ex vivo model identifies TLE4 as a co-repressor in T-ALL |
|
| Relations |
| BioSample |
SAMN41864677 |
| SRA |
SRX24943628 |
