|
| Status |
Public on Jun 26, 2024 |
| Title |
ko-962-2 |
| Sample type |
SRA |
| |
|
| Source name |
sNF96.2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: sNF96.2
cell type: Schwann Cell
genotype: TSPO knockdown
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cell lines and tumor tissues using the RNeasy Plus Mini Kit (Qiagen #74136)
The library was prepared using the Illumina® Stranded mRNA Prep kit followed by RNAseq on Illumina HiSeq platform
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Data processing |
Trimglore
Sequencing reads were mapped to human hg38 genome using HISAT2
Samtools sam to bam
Featurecount
Assembly: Hg38
Supplementary files format and content: Tab-delimited text file including raw counts for each samples
|
| |
|
| Submission date |
Jun 26, 2024 |
| Last update date |
Jun 26, 2024 |
| Contact name |
Chenhao Hu |
| E-mail(s) |
huchenhaotiantan@gmail.com
|
| Organization name |
Beijing Neurosurgical Institute
|
| Street address |
No.119 South Fourth Ring West Road, Fengtai District, Beijing, China
|
| City |
Beijing |
| ZIP/Postal code |
100000 |
| Country |
China |
| |
|
| Platform ID |
GPL18460 |
| Series (1) |
| GSE270880 |
TSPO deficiency promotes the progression of malignant peripheral sheath tumors by regulating the G2/M phase of the cell cycle via CDK1 |
|
| Relations |
| BioSample |
SAMN42088489 |
| SRA |
SRX25091010 |
