|
| Status |
Public on Jun 15, 2012 |
| Title |
pRS-kdm3a 72h 1 |
| Sample type |
RNA |
| |
|
| Source name |
F9 cells
|
| Organism |
Mus musculus |
| Characteristics |
kdm3a expression: Knock-down
cell line: F9
treatment: Retinoic acid during 3 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by the Tripure method (Roche) according to the manufacturer's instructions and purified on RNeasy minicolumns (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 200 ng of total RNA with the Illumina® TotalPrep RNA Amplification Kit (Ambion)
|
| |
|
| Hybridization protocol |
cRNA was hybridized on the MouseWG-6 v2 array according to the Whole-Genome Gene Expression Direct Hybridization Assay (Illumina)
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
biological replicate 1
Sample 7
|
| Data processing |
The data were normalized using quantile normalization with the 'beadarray' package in R
|
| |
|
| Submission date |
Nov 21, 2011 |
| Last update date |
Jun 15, 2012 |
| Contact name |
Sarah Dedeurwaerder |
| E-mail(s) |
sdedeurw@ulb.ac.be
|
| Organization name |
Université Libre de Bruxelles
|
| Lab |
Cancer Epigenetics
|
| Street address |
Route de Lennik, 808
|
| City |
Brussels |
| ZIP/Postal code |
1070 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE33841 |
The histone demethylase Kdm3a is essential to progression through differentiation |
|
