|
| Status |
Public on Jul 06, 2024 |
| Title |
Patient derived PBNs |
| Sample type |
SRA |
| |
|
| Source name |
Blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Blood
cell type: Neutrophils
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were purified via FACS as detailed above into Protector RNase Inhibitor, then validated for viability on a Countess automated cell counter.
Library preparation was performed as per manufacturer recommendations (single cell 3' v3 10x Genomics) for processing granulocytes.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Demultiplexing, barcoded processing, gene counting and aggregation were performed using the Cell Ranger software v6.1.2.
Assembly: GRCh38-2020-A
Supplementary files format and content: Tab-separated values files and matrix files
|
| |
|
| Submission date |
Jul 05, 2024 |
| Last update date |
Jul 06, 2024 |
| Contact name |
Manish Aghi |
| E-mail(s) |
manish.aghi@ucsf.edu
|
| Organization name |
UCSF
|
| Street address |
505 Parnassus Avenue, #M774A
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94117 |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE271618 |
Glioblastoma induces the recruitment and differentiation of hybrid neutrophils from skull bone marrow |
|
| Relations |
| BioSample |
SAMN42339969 |
| SRA |
SRX25221169 |
