|
| Status |
Public on Jul 20, 2024 |
| Title |
wild type embryo-1 |
| Sample type |
RNA |
| |
|
| Source name |
12 days embryo
|
| Organism |
Mus musculus |
| Characteristics |
tissue: embryo
strain: C57BL/6J
|
| Growth protocol |
The animal housing facility is a barrier housing facility, and it is maintained in accordance with the national standards (Laboratory Animal-Requirements of Environment and Housing Facilities, GB 14925–2001).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of tumor tissue were isolated using Trizol. RNA quantity and quality were measured by NanoDrop ND-1000. RNA integrity was assessed by standard denaturing agarose gel electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
total RNA from each sample was linearly amplified and labeled with Cy3-UTP.The Labeled cRNAs were purified by RNeasy Mini Kit (Qiagen). The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000.
|
| |
|
| Hybridization protocol |
1 μg of each labeled cRNA was fragmented by adding 11 μl 10 × Blocking Agent and 2.2 μl of 25×Fragmentation Buffer, then heated at 60°C for 30 min, and finally 55 μl 2 × GE Hybridization buffer was added to dilute the labeled cRNA. 100 μl of hybridization solution was dispensed into the gasket slide and assembled to the gene expression microarray slide. The slides were incubated for 17 hours at 65°C in an Agilent Hybridization Oven.
|
| Scan protocol |
The hybridized arrays were washed, fixed and scanned with using the Agilent DNA Microarray Scanner (part number G2505C).
|
| Data processing |
Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images.
|
| |
|
| Submission date |
Jul 07, 2024 |
| Last update date |
Jul 20, 2024 |
| Contact name |
LI XU |
| E-mail(s) |
xulihhb@gmail.com
|
| Phone |
13003691133
|
| Organization name |
Zhejiang Chinese Medical University
|
| Department |
School of Basic Medical Science
|
| Street address |
Binwen 7-3-1101,Binwen Road
|
| City |
HangZhou |
| State/province |
Zhejiang Province |
| ZIP/Postal code |
310053 |
| Country |
China |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE271656 |
Transcriptome analysis of embryos of STAT3-K177R/K180R mutant mice |
|
